Iron-Stimulated Production and Antimicrobial Potential of a Novel Biosurfactant Produced by a Drilling Waste-Degrading Pseudomonas citronellolis Strain

A Pseudomonas citronellolis strain was isolated from drilling waste (DW). This utilizes DW as the sole energy and carbon source to produce biosurfactants (BSs). The BS produced thermally stable, amorphous includes a peptide structure. FeSO4, FeCl3 Fe(NO3)3 were supplemented at various concentration levels assess possible enhancement of production biodegradation. limit Fe compounds between increase in formation microbial toxicity 0.1 mM. enhanced biodegradation more than doubled yield, determining an optimization strategy for production. then partially purified used against several Gram-negative positive multi-drug resistant bacteria (such Klebsiella pneumoniae, aeruginosa, Escherichia coli spp, Acinetobacter baumaniii, Enterococcus faecalis Streptococcus Staphylococcus aureus, Salmonella enterica). minimum inhibitory defined range 0.25 10 mg/mL. antimicrobial properties established its effectiveness suggested down-stream processing cost reduction, no additional purification steps necessary. study could lead sustainable low-cost bioprocess towards circular bioeconomy because waste, non-expensive substrate, is used; while holds great potential novel compound with antibiotic disinfectant-like action, following testing human cells.